Flatness of Dichroic Beamsplitters Affects Focus and Image Quality

In order to maximize transmission and reliability while minimizing artifacts associated with multiple surfaces...

Flatness of Dichroic Beamsplitters Affects Focus and Image Quality

By Semrock

 

Even though fluorescence microscopy has become a routine technique for many applications, demanding requirements from technological advances continue to push the limits. For example, today biological researchers are not only interested in looking at fixed-cell samples labeled with multiple colors, but they are also interested in looking at the dynamics of live specimens in multiple colors, simultaneously. Even certain fixed-cell imaging applications are hampered by limited throughput of the conventional multicolor imaging techniques. Given that the most popular multicolor fluorescence imaging configurations - including “Sedat” and “Pinkel” - are often too slow, new multicolor imaging approaches ...

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