Optical Filters for Laser-based Fluorescence Microscopes

Lasers have been advantageously replacing conventional broadband light sources for fluorescence imaging applications

Optical Filters for Laser-based Fluorescence Microscopes

By Semrock

 

A dynamically customizable solution for the microscope or optical table. Craig Hodgson, Ph.D. and Cicely Rathmell, M.Sc.

 


Due to many desirable properties such as high brightness, stability, longevity, and narrow spectral bandwidth, lasers have been advantageously replacing conventional broadband light sources for fluorescence imaging applications. Not only have these features of lasers allowed for higher-sensitivity visualization and enhanced throughput in imaging applications, but also several unique properties of lasers - including narrow beam divergence, high degree of spatial and temporal coherence, and well-defined polarization properties - have spawned new fluorescence imaging techniques. However, the advent of lasers as fluorescence light sources imposes new constraints on imaging systems and their components. For example, optical filters used in laser-based imaging systems such as confocal and Total Internal Reflection
Fluorescence (TIRF) microscopes have specific requirements that are unique compared to...

Download the Whitepaper