Filter Sets for Next Generation Fluorescence Microscopy - LED Filter Sets
Prashant Prabhat, Ph.D.
Ongoing improvements in performance and availability have earned solid-state LED light sources considerable attention and wider use in fluorescence microscopy and related fields. Features such as long lifetimes, rapid switching response, consistent intensity, and stable spectral and operation characteristics have helped make the LED an attractive alternative to earlier arc lamp and Xenon light sources. A new generation of light engines (LE) now use LED sources to generate the illumination needed for stimulating fluorescence from various types of tissue samples, fluorophores, and other materials. The new capabilities for precision spectral performance obtained from LED LEs have helped expand the use of fluorescence imaging to an ever increasing range of medical, biological, and other research applications. As new LED sources are developed and gain acceptance, there are corresponding changes taking place in optical filters. But these changes...
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