Fluorescence Polarization in Life Sciences

By Semrock

Turan Erdogan, Ph.D.

The application of fluorescence polarization offers unique advantages over conventional fluorescence imaging and quantitation. In fluorescence polarization studies, fluorophores tied to biological samples or chemical compounds help elucidate the underlying mechanism of interest. As opposed to the standard fluorescence techniques, fluorescence polarization allows for fast and accurate quantitative measurements with relatively simple instrumentation (compared to sophisticated research instruments). Therefore, applications such as High Throughput Screening (HTS), a key technique in drug discovery, have made widespread use of fluorescence polarization. Progressively more complicated studies at the molecular level are being conducted by the application of microscopy-based fluorescence polarization studies.
Excellent technical information on the theory and applications of fluorescence polarization is available in the literature [1-6]. However many of these resources provide a limited perspective on applications. ...

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