509 nm edge BrightLine® single-edge image-splitting dichroic beamsplitter for super-resolution microscopy

Product Code Description Lead Time Price Quantity Buy / Get Quote
FF509-FDi02-t3-25x36 509 nm edge BrightLine® single-edge image-splitting dichroic beamsplitter for super-resolution microscopy 1-2 weeks £588.00
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Angle of Incidence:

45 ± 1.5 °

Clear Aperture:

≥ 80% (elliptical)

Common Fluorophore Pair to Split:


Cone Half-angle:

Edge Wavelength:


Filter Effective Index:

1.87 Understanding 'Effective Index of Refraction' neff

Filter Thickness Tolerance, Unmounted:

± 0.1mm

Filter Thickness, Unmounted:



λ/5 P-V RWE @ 632.8

Flatness / RWE Classification:


Mounted / Unmounted:


Optical Damage Rating:

Testing has proven to show no signs of degradation when exposed to at least 6.0 W of power from an unfiltered xenon arc lamp over a 25 mm diameter (corresponding to 1.2 W/cm2) for over 500 hrs.


Reflective surface marked with laser dot – Orient in direction of incoming light

Product Type:

Single-edge Dichroic Beamsplitter

Reflected Wavefront Error:

< 0.2λ P-V RWE @ 632.8

Reflection Band:

Ravg > 95% 350 – 500 nm

Semrock Filter Family:

BrightLine® Fluorescence Filters




25.2 x 35.6 x 3.0 mm



Substrate Thickness (3 mm, Unmounted):

3.0 mm

Substrate Type:

low-autofluorescence optical quality glass

Surface Quality (Scratch-Dig):


Transmission Band:

Tavg > 93% 518.3 – 1200 nm

Transverse Dimensions, LxW:

25.2 mm x 35.6 mm

Transverse Tolerance, Mounted:

± 0.1 mm

BrightLine® image-splitting dichroic beamsplitters offer superb image quality for both transmitted and reflected light when separating beams of light by color for simultaneous capture of multi-color images. For applications such as (FRET) and real-time live-cell imaging, users can now separate two, four or even more colors onto as many cameras or regions of a single camera sensor. The exceptional flatness of these filters virtually eliminates aberrations in the reflected beam for most common imaging systems.

Semrock's image-splitting dichroics for super-resolution microscopy deliver industry-leading λ/5 P-V RWE on 3 mm thick substrates for minimal focus shift and optical wavefront aberrations of the reflected imaging beam. These dichroics can be used with much larger diameter imaging beams up to 37 mm while minimizing RWE in custom size versions.

Common Fluorophore Pair to Split: CFP/YFP

FF509-FDi01 image-splitting dichroic beamsplitter for standard micrscopy available on 1 mm substrate for reflecting imaging beams up to 10 mm in diameter while minimizing RWE


Note on spectral plots:
In the past, each shipment has included a spectral plot for every filter and every lot code in the shipment. In an effort to be more eco-friendly, we will no longer be including these plots with each shipment. If you require spetral plot data, please see here for more information.