594 nm laser BrightLine® single-edge super-resolution / TIRF dichroic beamsplitter

Product Code Description Lead Time Price Quantity Buy / Get Quote
Di03-R594-t1-25x36 594 nm laser BrightLine® single-edge super-resolution / TIRF dichroic beamsplitter 1-2 weeks £509.00
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Angle of Incidence:

45 ° with a shift of 0.35%/° (40 – 50 °)

Clear Aperture:

≥ 80% (elliptical)

Cone Half-angle:


Filter Effective Index:

2.09 Understanding 'Effective Index of Refraction' neff

Filter Thickness Tolerance, Unmounted:

± 0.05mm

Filter Thickness, Unmounted:



λ/5 P-V RWE @ 632.8


1λ P-V RWE @ 632.8

Flatness / RWE Classification:

Super-resolution / TIRF

Laser Wavelength:


Laser Wavelength:

594.0nm ± 0.3

Laser Wavelength:


Mounted / Unmounted:


Optical Damage Rating:

1 J/cm2 @ 532 nm (10 ns pulse width)

Product Type:

Single-edge Laser Dichroic Beamsplitter

Reflected Wavefront Error:

< 0.2λ P-V RWE @ 632.8

Reflected Wavefront Error:

< 1λ P-V RWE @ 632.8

Reflection Band:

Rabs > 94% 593.5 – 594.3 nm

Reflection Band, p-pol:

Rabs > 90% 593.5 – 594.3 nm

Reflection Band, s-pol:

Rabs > 98% 593.5 – 594.3 nm

Semrock Filter Family:

BrightLine® Fluorescence Filters




25.2 x 35.6 x 1.1 mm

Substrate Thickness (1 mm, Unmounted):

1.05 mm

Substrate Thickness Tolerance (1 mm, Unmounted):

± 0.05 mm

Substrate Type:

low-autofluorescence optical quality glass

Surface Quality (Scratch-Dig):


Transmission Band:

Tavg > 93% 605 – 1200 nm

Transverse Dimensions, LxW:

25.2 mm x 35.6 mm

Transverse Tolerance, Mounted:

± 0.1 mm

The perfect dichroic beamsplitters for the most popular lasers used in fluorescence imaging, including all-solid-state lasers for advanced microscopy. All beamsplitters in this category have exceptional reflectance at the key laser wavelengths, and wider reflection bands — into UV for photoactivation and super-resolution techniques. Additionally they feature extended transmission regions — into IR to 1200 or 1600 nm, and anti-reflection (AR) coatings to minimize imaging artifacts resulting from the coherent laser light.

Semrock's super-resolution / TIRF dichroics are available in two thicknesses and deliver industry-leading flatness for minimal focus shift and optical wavefront aberrations of the laser beam spot to enable popular imaging and Super-resolution techniques such as TIRF, PALM, STORM, Structured Illumination, and STED.

1λ P-V RWE on 1 mm, optimized for reflecting laser beams up to 10 mm in diameter while minimizing RWE
λ/5 P-V RWE on 3 mm, optimized for reflecting laser beams up to 22.5 mm in diameter while minimizing RWE


Note on spectral plots:
In the past, each shipment has included a spectral plot for every filter and every lot code in the shipment. In an effort to be more eco-friendly, we will no longer be including these plots with each shipment. If you require spetral plot data, please see here for more information.