Di03-R405/488/561/635-t1-25x36

405/488/561/635 nm lasers BrightLine® quad-edge super-resolution / TIRF dichroic beamsplitter

Product Code Description Lead Time Price Quantity Buy / Get Quote
Di03-R405/488/561/635-t1-25x36 405/488/561/635 nm lasers BrightLine® quad-edge super-resolution / TIRF dichroic beamsplitter 1-2 weeks £534.00
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Specifications

Angle of Incidence:

45 ° with a shift of 0.35%/° (40 – 50 °)

Clear Aperture:

≥ 80% (elliptical)

Cone Half-angle:

0.5°

Filter Effective Index:

2.05 Understanding 'Effective Index of Refraction' neff

Filter Thickness Tolerance, Unmounted:

± 0.05mm

Filter Thickness Tolerance, Unmounted:

± 0.1mm

Filter Thickness, Unmounted:

1.05mm

Filter Thickness, Unmounted:

3.0mm

Flatness (3 mm):

1/5 wave PV RWE @ 632.8 on 3 mm

Flatness / RWE Classification:

Super-resolution / TIRF

Mounted / Unmounted:

Unmounted

Optical Damage Rating:

1 J/cm2 @ 532 nm (10 ns pulse width)

Product Type:

Quad-edge Laser Dichroic Beamsplitter

Reflected Wavefront Error:

< 0.2λ P-V RWE @ 632.8

Reflected Wavefront Error:

< 1λ P-V RWE @ 632.8

Semrock Filter Family:

BrightLine® Fluorescence Filters

Shape:

rectangular

Size:

25.2 x 35.6 x 1.1 mm

Substrate Thickness (1 mm, Unmounted):

1.05 mm

Substrate Thickness (3 mm, Unmounted):

3.0 mm

Substrate Thickness Tolerance (1 mm, Unmounted):

± 0.05 mm

Substrate Type:

low-autofluorescence optical quality glass

Surface Quality (Scratch-Dig):

60-40

Transverse Dimensions, LxW:

25.2 mm x 35.6 mm

Transverse Tolerance, Mounted:

± 0.1 mm

Band 1

Edge Wavelength:

418.7nm

Laser Wavelength:

375nm ± 3

Laser Wavelength:

405nm ± 5

Reflection Band:

Rabs > 94% 370 – 410 nm

Reflection Band, p-pol:

Rabs > 90% 370 – 410 nm

Reflection Band, s-pol:

Rabs > 98% 370 – 410 nm

Transmission Band:

Tavg > 93% 426.0 – 462.0 nm

Band 2

Edge Wavelength:

498.3nm

Laser Wavelength:

473nm +2/-0

Laser Wavelength:

488nm +3/-2

Reflection Band:

Rabs > 94% 473 – 491 nm

Reflection Band, p-pol:

Rabs > 90% 473 – 491 nm

Reflection Band, s-pol:

Rabs > 98% 473 – 491 nm

Transmission Band:

Tavg > 93% 502.5 – 544.5 nm

Band 3

Edge Wavelength:

575.4nm

Laser Wavelength:

559nm +5/-0

Laser Wavelength:

561.4nm

Laser Wavelength:

568.2nm

Reflection Band:

Rabs > 94% 559 – 568.2 nm

Reflection Band, p-pol:

Rabs > 90% 559 – 568.2 nm

Reflection Band, s-pol:

Rabs > 98% 559 – 568.2 nm

Transmission Band:

Tavg > 93% 582 – 617.5 nm

Band 4

Edge Wavelength:

655.3nm

Laser Wavelength:

632.8nm

Laser Wavelength:

635nm +7/-0

Laser Wavelength:

647.1nm

Reflection Band:

Rabs > 94% 632.8 – 647.1 nm

Reflection Band, p-pol:

Rabs > 90% 632.8 – 647.1 nm

Reflection Band, s-pol:

Rabs > 98% 632.8 – 647.1 nm

Transmission Band:

Tavg > 93% 663 – 1200 nm

Band 5

Reflection Band:

Ravg > 90% 350 – 370 nm

The perfect beamsplitters for the most popular lasers used in fluorescence imaging, including all-solid-state lasers. All beamsplitters in this category have exceptional reflectance at the key laser wavelengths, and wider reflection bands — into UV for photoactivation and super-resolution techniques. Additionally they feature extended transmission regions — into IR to 1200 or 1600 nm, and anti-reflection (AR) coatings to minimize imaging artifacts resulting from the coherent laser light.

Semrock's super-resolution / TIRF dichroics are available in two thicknesses and deliver industry-leading flatness for minimal focus shift and optical wavefront aberrations of the laser beam spot to enable popular imaging and Super-resolution techniques such as TIRF, PALM, STORM, Structured Illumination, and STED.

1λ P-V RWE on 1 mm, optimized for reflecting laser beams up to 10 mm in diameter while minimizing RWE
λ/5 P-V RWE on 3 mm, optimized for reflecting laser beams up to 22.5 mm in diameter while minimizing RWE