Di01-R405/488/561/635/800-t3-25x36

405/488/561/635/800-1050 nm lasers BrightLine® multi-edge multiphoton super-resolution / TIRF dichroic beamsplitter

Product Code Description Lead Time Price Quantity Buy / Get Quote
Di01-R405/488/561/635/800-t3-25x36 405/488/561/635/800-1050 nm lasers BrightLine® multi-edge multiphoton super-resolution / TIRF dichroic beamsplitter 1-2 weeks £893.00
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Specifications

Angle of Incidence:

45 ° with a shift of ~0.35%/° (40 – 50 °)

Clear Aperture:

= 80% (elliptical)

Cone Half-angle:

0.5°

Filter Effective Index:

1.87 Understanding 'Effective Index of Refraction' neff

Filter Thickness Tolerance, Unmounted:

± 0.05mm

Filter Thickness Tolerance, Unmounted:

± 0.1mm

Filter Thickness, Unmounted:

1.05mm

Filter Thickness, Unmounted:

3.0mm

Flatness / RWE Classification:

Super-resolution / TIRF

Group Delay Dispersion:

± 500 fs2 over 800 - 1050 nm reflection band for S-Pol and P-Pol

Mounted / Unmounted:

Unmounted

Optical Damage Rating:

1 J/cm2 @ 532 nm (10 ns pulse width)

Reflected Wavefront Error:

< 0.33λ P-V RWE @ 632.8

Reflected Wavefront Error:

< 2.5λ P-V RWE @ 632.8

Semrock Filter Family:

BrightLine® Fluorescence Filters

Shape:

rectangular

Size:

25.2 x 35.6 x 3.0 mm

Substrate Thickness (1 mm, Unmounted):

1.05 mm

Substrate Thickness (3 mm, Unmounted):

3.0 mm

Substrate Thickness Tolerance (1 mm, Unmounted):

± 0.05 mm

Substrate Type:

low-autofluorescence optical quality glass

Surface Quality (Scratch-Dig):

60-40

Transverse Dimensions, LxW:

25.2 mm x 35.6 mm

Transverse Tolerance, Mounted:

± 0.1 mm

Band 1

Edge Wavelength:

420nm

Laser Wavelength:

405nm ± 5

Reflection Band:

Rabs > 94% 400 – 410 nm

Reflection Band, p-pol:

Rabs > 90% 400 – 410

Reflection Band, s-pol:

Rabs > 98% 400 – 410

Transmission Band:

Tavg > 93% 425 – 470 nm

Band 2

Edge Wavelength:

501nm

Laser Wavelength:

488nm ± 5

Reflection Band:

Rabs > 94% 483 – 493 nm

Reflection Band, p-pol:

Rabs > 90% 483 – 493

Reflection Band, s-pol:

Rabs > 98% 483 – 493

Transmission Band:

Tavg > 93% 508 – 540 nm

Band 3

Edge Wavelength:

577nm

Laser Wavelength:

559nm +4/-0

Laser Wavelength:

561.4nm

Reflection Band:

Rabs > 94% 559 – 563 nm

Reflection Band, p-pol:

Rabs > 90% 559 – 563

Reflection Band, s-pol:

Rabs > 98% 559 – 563

Transmission Band:

Tavg > 93% 583 – 615 nm

Band 4

Edge Wavelength:

662nm

Laser Wavelength:

635nm +7/-0

Laser Wavelength:

642nm ± 5

Reflection Band:

Rabs > 94% 635 – 647 nm

Reflection Band, p-pol:

Rabs > 90% 635 – 647

Reflection Band, s-pol:

Rabs > 98% 635 – 647

Transmission Band:

Tavg > 93% 671 – 725 nm

Band 5

Edge Wavelength:

735nm

Laser Wavelength:

800 - 1050nm

Reflection Band:

Ravg > 94% 800 – 1050 nm

Reflection Band, p-pol:

Ravg > 90% 800 – 1050

Reflection Band, p-pol:

Ravg > 98% 800 – 1050

Innovative dichroic beamsplitter designed to simplify the combination of 1P confocal imaging and 2P excitation for multiphoton applications. This dichroic beamsplitter includes reflection bands for the most popular lasers used in fluorescence imaging, including all-solid-state lasers. The 405 nm reflection is optimal for imaging or uncaging applications. The 2P excitation region is ideal for femtosecond pulsed lasers such as Ti:Sapphire, OPO coupled, and Ytterbium-doped lasers, enabling deep tissue imaging with improved constrast. These multiphoton dichroics have low reflected GDD that helps minimize temporal pulse broadening of reflected laser beams, and have steep edges with very high and flat reflection and transmission bands.

Semrock's super-resolution / TIRF dichroics are available in two thicknesses and deliver industry-leading flatness for minimal focus shift and optical wavefront aberrations of the laser beam spot to enable popular imaging and super-resolution techniques such as TIRF, PALM, STORM, structured illumination, and STED.

2.5λ P-V RWE on 1 mm, optimized for reflecting laser beams up to 6 mm in diameter while minimizing RWE
λ/3 P-V RWE on 3 mm, optimized for reflecting laser beams up to 16.7 mm in diameter while minimizing RWE