Innovative dichroic beamsplitter designed to simplify the combination of 1P confocal imaging and 2P excitation for multiphoton applications. This dichroic beamsplitter includes reflection bands for the most popular lasers used in fluorescence imaging, including all-solid-state lasers. The 405 nm reflection is optimal for imaging or uncaging applications. The 2P excitation region is ideal for femtosecond pulsed lasers such as Ti:Sapphire, OPO coupled, and Ytterbium-doped lasers, enabling deep tissue imaging with improved constrast. These multiphoton dichroics have low reflected GDD that helps minimize temporal pulse broadening of reflected laser beams, and have steep edges with very high and flat reflection and transmission bands.
Semrock's super-resolution / TIRF dichroics are available in two thicknesses and deliver industry-leading flatness for minimal focus shift and optical wavefront aberrations of the laser beam spot to enable popular imaging and super-resolution techniques such as TIRF, PALM, STORM, structured illumination, and STED.
2.5λ P-V RWE on 1 mm, optimized for reflecting laser beams up to 6 mm in diameter while minimizing RWE
λ/3 P-V RWE on 3 mm, optimized for reflecting laser beams up to 16.7 mm in diameter while minimizing RWE