DAPI-1160B-L01-ZERO

BrightLine single-band zero pixel shift filter set (Leica "Large"): DAPI and similar fluorophores

Product Code Description Lead Time Price Quantity Buy / Get Quote
DAPI-1160B-L01-ZERO BrightLine single-band zero pixel shift filter set (Leica "Large"): DAPI and similar fluorophores 8-10 weeks £784.00
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Specifications

Blocking:

BrightLine filters have blocking far exceeding OD 6 as needed to ensure a black background, even when using modern low-noise CCD cameras. The blocking is optimised for microscopy applications using the SpecMaker™ fluorescence filter design

Dichroic Orientation:

Reflective coating side should face toward light source and sample.

Exciter/Emitter Orientation:

Arrow on ring indicates preferred direction of propagation of light.

Filter set type:

Single-band

Filter size:

Leica "Large"

Fluorophore:

Alexa Fluor 350

Fluorophore:

AMCA

Fluorophore:

BFP

Fluorophore:

DAPI

Fluorophore:

Hoechst 33258

Fluorophore:

Hoechst 33342

Fluorophore:

Hoechst 34580

Fluorophore:

LysoSensor Blue

Fluorophore:

Marina Blue

Fluorophore:

Pacific Blue

Fluorophore:

sgBFP

Microscope Compatibility:

BrightLine filters are available to fit Leica, Nikon, Olympus, Zeiss, and Aperio microscopes.

Optical Damage Rating:

Testing has proven to show no signs of degradation when exposed to at least 6.0 W of power from an unfiltered xenon arc lamp over a 25 mm diameter (corresponding to 1.2 W/cm2) for over 500 hrs.

Surface Quality (Scratch-Dig):

60-40

Zero pixel shift:

yes

Highest Contrast DAPI set on the market. The preferred DAPI set for most applications, this filter set is optimized for achieving exceptionally low noise (background) yet high brightness, resulting in unsurpassed contrast (signal-to-noise ratio). Images of typical cell samples with DAPI-stained nuclei exhibit more than twice the contrast of those made with the leading competitor's set. The carefully optimized narrow, red-shifted exciter filter minimizes fluorophore photobleaching and sample phototoxicity. What's more, unlike soft-coated filters and absorbing glass filters used for DAPI excitation, the unique hard-coated exciter does not "burn-out" under the intense UV illumination of modern fluorescence light sources.

This set is the unparalleled choice for most situations, especially those involving digital imaging. For specialized applications where brightness trumps contrast, use our DAPI-5060C set, which has the highest brightness on the market.

 

Note on spectral plots:
In the past, each shipment has included a spectral plot for every filter and every lot code in the shipment. In an effort to be more eco-friendly, we will no longer be including these plots with each shipment. If you require spetral plot data, please see here for more information.