What are the benefits of using a fixed Femtosecond Fibre Laser in Two-Photon microscopy?

Find out why more people are choosing them as an alternative to Ti:Sapphire lasers.

Written by our Femtosecond Fibre Laser specialist, Karen McGeachy,  this blog is aimed to help you understand the benefits that fixed wavelength femtosecond fibre lasers offer to your two-photon microscopy set-up. Click on the image below to download the full PDF.


We will cover the following topics:


  • Brief overview of two-photon microscopy
    • This section details the principle of two-photon microscopy and why it is used.
  • Ti:Sapphire lasers, the traditional excitation laser
    • This section provides an overview of the traditional two-photon laser and why it was suitable.
  • Can fibre lasers be used as an alternative?
    • This section explores the feasibility of using a femtosecond fibre laser in two-photon microscopy.
  • Why should people choose fibre lasers?
    • This section provides a comparison of Femtosecond Fibre Lasers vs Ti:Sapphire lasers and explores the difference in wavelength and power, price, and maintenance and form factor.
  • Key specifications of the SPARK LASERS ALCOR series for two-photon microscopy
    • This section goes into details of the features of the ALCOR which makes it particularly beneficial for two-photon microscopy such as GDD precompensation, adjustable power and triggering, and fibre coupling.