Agilent MLC400 Monolithic Laser Combiner: Laser Engine for Microscopy / Cell Biology

The only laser engine for confocal microscopy that will never need alignment.

The Agilent Technologies MLC400 monolithic laser combiner is a revolutionary compact multi-line laser engine designed for fluorescence and confocal microscopy research applications. Currently offering up to four wavelengths, applications include: TIRF FISH FRAP FLIM FRET Super-resolution

The Agilent CMO technology and fiber delivery system are at the heart of the MLC400

No maintenance, no cleaning, and no engineers are required, which dramatically reduces downtime.

Multiple beam-combining optics are assembled into a single optical structure using Agilent's proprietary complex monolithic optic (CMO) technology that allows them to be permanently aligned before they leave the factory. A proprietary fixed mounting system keeps the beam-steering optics permanently aligned and the beam is delivered to the microscope by a single-mode, polarization-maintaining fibre-coupled delivery system.

This design reduces the number of exposed beam-combination surfaces that are subject to contamination. Full integration of the combining optics and beam steering optics into the beam delivery architecture removes the possibility of contamination and therefore the need to clean.

The system does not require an engineer for installation. This plug and play simplicity means that demo equipment can even be brought to you. Please contact us for details.

Consistent results, ideal when you need to quantify fluorescence.

The permanently aligned optics and compact one piece chassis means that environmental changes that commonly occur in laboratories, such as temperature, air flow, and benchtop vibration, are no longer an issue. By providing stable illumination, it minimises cell exposure to light and extends the imaging time. The inclusion of the microprocessor controller reduces photobleaching further.

The lasers and components in the system are built by leading manufacturers and combined with Agilent's proprietary parts deliver a highly efficient and stable product, whether you require long term imaging or fast acquisition rates. This combination gives long-term stability, so that the power delivered remains constant day after day, result after result.

See below for the output power, as delivered to your instrument from the tip of the light guide.

Feeling overcrowded?

The single unit design of the Agilent laser engine makes the system compact, compared to other multi-laser systems. It fits on a desktop and is easily relocated without the need for adjustment or alignment.

Synchronise / integrate fully with your imaging system.

Via the onboard DSP microcontroller, the MLC400 is fully integrated into your complex imaging system, whether triggered by your camera or driving your stage. This includes both simple event triggers and user defined command sequences, to allow device-to-device control that bypasses the host computer/software.

Precise control for all advanced imaging techniques.

Independent on/off control of each laser extends the laser lifetime. An acousto-optic tunable filter (AOTF) provides fast switching between different combinations of wavelengths and powers.

The result is a laser light source for cell biology that is stable, reliable and easy-to-use.