LED based light engine for fluorescence microscopy

One microscope light source solution for all of your current and future fluorescent illumination needs.

The Spectra X light engine contains six discrete outputs, to cover the whole visible spectrum. Combined with exchangeable filter modules, you have every wavelength for every fluorophor you need; now and in the future.

These solutions are not just microscope light sources. They are light engines. These systems replace the entire ensemble of light sources, excitation filters, filter wheel, field diaphragm, aperture, shutter and controllers with a single, simple, easy to operate unit.

Unlike traditional lamps, the output from the Spectra light engine does not decay or fluctuate significantly with time, or during the course of a multi-day experiment.

A flexible solution for shared facilities, the Spectra light engine gives you precise control.

The Spectra light engine range matches or beats the power levels of comparable metal halide and xenon lamps. In addition, precise intensity control allows you to select the ideal level for you work. This gives you the right light exposure, whether for simple immuno fluorescence or a complex live cell experiment.

Lumencor light engines are true replacements for traditional microscope light sources. They are able to output one, several or all channels. This functionality even allows you to use the light engine for visual work in combination with multi-bandpass filters, or for imaging techniques that require simultaneous excitation of multiple fluorophores.

A remote control accessory (RCA) gives you simple stand-alone control. This lets you look at a sample quickly, without fuss. The controller also provides you with advanced control through pre-programmable sequences.

Protect your samples

A primary concern of the biological microscopist is sample viability and well-being. Delivering too much light to a sample can cause phototoxic events. These interfere with normal cellular processes and can result in premature cell death. Too much light can also cause photobleaching of fluorophores. This limits the duration of otherwise long term imaging experiments.

The use of solid-state LED technology eliminates stray UV and IR light that can damage cells. The fact that most filters do not block UV light is no longer a problem. In this way, the spectrally pure outputs already reduce photo-toxic events that occur while imaging samples.

For further protection, the Camera Interface Accessory (CIA) takes the SPECTRA's timing and control to the next level of precision. The CIA allows the SPECTRA to run as master or slave in an imaging system. This direct control ensures the SPECTRA is perfectly synchronised with the camera and that the biological samples are exposed to the minimum amount of light needed for a given experiment.

Spend more time on the important aspects of your work

The light engine is always ready to use. The system never requires alignment or adjustments, and warms up to a stable state in seconds. The light engine always produces an aligned, uniform field of illumination across your entire sample. In addition, valuable time is not lost to bulb changes or maintenance.

  • Six outputs with interchangeable filter paddles to address existing and future fluorophores, NIR options available
  • Spectral & power stability
  • Spectral purity, no out of band light
  • Completely flat illumination
  • Microsecond switching of wavelength and intensity - faster than any filter wheel.
  • No external filters, shutters
  • Quiet and no heat generation
  • Ideal for electrophysiology & systems sensitive to electrical noise
  • Controllable by common microscopy automation software
  • Long life > 15,000 hours and no maintenance costs
  • Easy to use
  • 36 month warranty with guaranteed output levels




Product Datasheet Description
Quotation
SpectraX datasheet 6 Outputs with interchangeable filter paddles
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Spectra 3 – 7 Outputs with the ability to add channels post purchase
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RCA datasheet Keypad for control of Spectra X Light engines without the need for software
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CIA datasheet Camera interface accessory for synchronisation of the light engines with complex imaging systems
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OemX Talk to us about fully customised solutions for specialist and OEM applications.
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Connectivity datasheet Range of microscope adaptors and fibre sizes to allow use with your device.
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SOLA datasheet Solid state white light source
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Colour Example Fluor Output with Excitation Filters Light Engine Output Power  
1mm fibre 3mm LLG Microscope Collimator
Violet
380-410nm
DAPI /
Hoechst
390 / 18
386 / 23
379 / 34
80 mW
60 mW
50 mW
200 mW
150 mW
130 mW
250 mW
175 mW
150 mW

Power & Intensity Levels.pdf
Blue
420-455nm
CFP 438 / 24
434 / 17
445 / 20
105 mW
85 mW
60 mW
275 mW
230 mW
160 mW
350 mW
280 mW
190 mW
Cyan
460-490nm
GFP /
FITC
475 / 28
485 / 20
475 / 35
75 mW
45 mW
80 mW
205 mW
125 mW
215 mW
245 mW
140 mW
255 mW
Teal
500-520nm
YFP 513 / 17
510 / 10
512 / 25
20 mW
15 mW
25 mW
65 mW
40 mW
75 mW
90 mW
55 mW
100 mW
Green
535-600nm
TRITC /
Cy3
549 / 15
542 / 27
560 / 25
95 mW
110 mW
100 mW
260 mW
390 mW
380 mW
695 mW
705 mW
865 mW
Yellow
535-600nm
mCherry 575 / 25
586 / 20
585 / 29
100 mW
75 mW
80 mW
300 mW
200 mW
210 mW
660 mW
650 mW
650 mW
Red
620-750nm
Cy5 632 / 22
628 / 40
650 / 13
65 mW
70 mW
30 mW
180 mW
190 mW
105 mW
225 mW
235 mW
115 mW
NIR Output information will be available soon, please enquire

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